M30 Apoptosense and M65 ELISA (CE-IVD)

M30-Apoptosense® ELISA (Prod. No. 10010)

M30-Apoptosense® is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of caspase-cleaved Keratin-18 (ccK18, K18F, K18Asp396-NE: M30 neo-epitope) levels in cytosolic cell extracts, cell culture supernatants, human serum or plasma or other body fluids. The M30-Apoptosense® ELISA uses the M30 antibody which is specific to this K18 cleavage site and does not react with viable or necrotic cells.
The M30-Apoptosense® ELISA measures the accumulation of a caspase-generated product resulting in an unmatched sensitivity thus allowing the tracing of apoptotic cells of epithelial origin in patient plasma/serum. The most important applications are cancer and liver diseases.

The M65 EpiDeath® ELISA is the next generation of Peviva's biomarkers. It is intended for the quantitative detection of keratin 18 (K18) present in cell culture supernatants, human serum, human plasma or other body fluids after it has been released from dying cells into the extracellular compartment. The assays offers the opportunity to quantify total cell death (due to apoptosis and necrosis) of K18 positive (epithelial) cells.

The M65 EpiDeath® ELISA, when used in combination with the M30 Apoptosense® ELISA (which specifically measures apoptosis), allows to quantify and distinguish different forms of epithelial cell death as both assay are standardised against the identical reference (Kramer et al., 2004).

The M65 EpiDeath® ELISA is a modified version of the M65® ELISA and is intended for the same applications. Further information about the similarities and differences between these two assaysis available on the website Comparison between M65 EpiDeath® ELISA and M65® ELISA.

The M65 EpiDeath® ELISA is registered as a medical device for in vitro diagnostic use in accordance with the CE-IVD directive.

M65® ELISA (Prod. No. 10020)

The M65® ELISA is intended for the quantitative detection of Keratin 18 (K18) present in cell culture supernatants, human serum, human plasma or other body fluids after it has been released from dying cells into the extracellular compartment. The M65® ELISA offers the opportunity to quantify total cell death (due to apoptosis and necrosis) of K18 positive (epithelial) cells.
The M65® ELISA when used in combination with the M30-Apoptosense® assay (which specifically measures apoptosis) allows to quantify and distinguish different forms of epithelial cell death as both assays are standardized against the identical reference.